Science
Scientists Map Miles of Wiring in a Speck of Mouse Brain
The human brain is so complex that scientific brains have a hard time making sense of it. A piece of neural tissue the size of a grain of sand might be packed with hundreds of thousands of cells linked together by miles of wiring. In 1979, Francis Crick, the Nobel-prize-winning scientist, concluded that the anatomy and activity in just a cubic millimeter of brain matter would forever exceed our understanding.
“It is no use asking for the impossible,” Dr. Crick wrote.
Forty-six years later, a team of more than 100 scientists has achieved that impossible, by recording the cellular activity and mapping the structure in a cubic millimeter of a mouse’s brain — less than one percent of its full volume. In accomplishing this feat, they amassed 1.6 petabytes of data — the equivalent of 22 years of nonstop high-definition video.
“This is a milestone,” said Davi Bock, a neuroscientist at the University of Vermont who was not involved in the study, which was published Wednesday in the journal Nature. Dr. Bock said that the advances that made it possible to chart a cubic millimeter of brain boded well for a new goal: mapping the wiring of the entire brain of a mouse.
“It’s totally doable, and I think it’s worth doing,” he said.
More than 130 years have passed since the Spanish neuroscientist Santiago Ramón y Cajal first spied individual neurons under a microscope, making out their peculiar branched shapes. Later generations of scientists worked out many of the details of how a neuron sends a spike of voltage down a long arm, called an axon. Each axon makes contact with tiny branches, or dendrites, of neighboring neurons. Some neurons excite their neighbors into firing voltage spikes of their own. Some quiet other neurons.
Human thought somehow emerges from this mix of excitation and inhibition. But how that happens has remained a tremendous mystery, largely because scientists have been able to study only a few neurons at a time.
In recent decades, technological advances have allowed scientists to start mapping brains in their entirety. In 1986, British researchers published the circuitry of a tiny worm, made up of 302 neurons. In subsequent years, researchers charted bigger brains, such as the 140,000 neurons in the brain of a fly.
Could Dr. Crick’s impossible dream be possible after all? In 2016, the American government began a $100 million effort to scan a cubic millimeter of a mouse brain. The project — called Machine Intelligence from Cortical Networks, or MICrONS — was led by scientists at the Allen Institute for Brain Science, Princeton University and Baylor College of Medicine.
The researchers zeroed in on a portion of the mouse brain that receives signals from the eyes and reconstructs what the animal sees. In the first stage of the research, the team recorded the activity of neurons in that region as it showed a mouse videos of different landscapes.
The researchers then dissected the mouse brain and doused the cubic millimeter with hardening chemicals. Then they shaved off 28,000 slices from the block of tissue, capturing an image of each one. Computers were trained to recognize the outlines of cells in each slice and link the slices together into three-dimensional shapes. All told, the team charted 200,000 neurons and other types of brain cells, along with 523 million neural connections.
For Nuno da Costa, a biologist at the Allen Institute and one of the leaders of the project, just watching the cells take shape on his computer screen was breathtaking. “These neurons are absolutely stunning — it gives me pleasure,” he said.
To understand how this mesh of neurons functioned, Dr. da Costa and his colleagues mapped the activity that had been recorded when the mouse looked at videos.
“Imagine that you come to a party that has 80,000 people, and you can be aware of every conversation, but you don’t know who is talking to whom,” Dr. da Costa said. “And now imagine that you have a way to know who is talking to whom, but you have no idea what they’re saying. If you have these two things, you can tell a better story about what’s happening at the party.”
Analyzing the data, the researchers discovered patterns in the wiring of the brain that had escaped notice until now. They identified distinct kinds of inhibitory neurons, for instance, that link only to certain other types of neurons.
“When you go into studying the brain, it seems kind of hopeless — there are just so many connections and so much complexity,” said Mariela Petkova, a biophysicist at Harvard who was not involved in the MICrONS project. “Finding wiring rules is a win. The brain is a lot less messy than people thought,” she said.
Many of the MICrONS researchers are now pitching in on a bigger project: mapping an entire mouse’s brain. With a volume of 500 cubic millimeters, a full brain would take decades or centuries to chart with current methods. The scientists will have to find additional tricks in order to finish the project in a decade.
“What they’ve already had to do to get here is heroic,” said Gregory Jefferis, a neuroscientist at the University of Cambridge who was not involved in the MICrONS project. “But we’ve still got a mountain to climb.”
Forrest Collman, a member of the MICrONS project at the Allen Institute, is optimistic. He and his colleagues recently discovered how to make microscopically thin sections from an entire mouse brain. “Some of these barriers are starting to fall,” Dr. Collman said.
But our own brain, which is about a thousand times bigger than a mouse’s, presents a much bigger challenge, he added. “The human brain right now feels like outside the range of what is possible,” he said. “We are not going there anytime soon.”
But Sebastian Seung, a neuroscientist at Princeton and a member of the MICrONS project, noted that mouse brains and human brains are similar enough that researchers might glean clues that could help them find medications to effectively treat psychological disorders without causing harmful side effects.
“Our current methods of manipulating the nervous system are incredibly blunt instruments,” Dr. Seung said. “You put in a drug, and it goes everywhere,” he added. “But being able to actually reach in and manipulate a cell type — that’s precision.”
The efforts to map a whole mouse brain are supported by funding from a long-running National Institutes of Health program called the BRAIN initiative. But the future of the endeavor is uncertain. Last year, Congress cut funding to the BRAIN initiative by 40 percent, and last month President Trump signed a bill cutting support by another 20 percent.
Dr. Bock noted that brain-mapping efforts like MICrONS take years, partly because they require the invention of new technologies and software along the way.
“We need consistency and predictability of science funding to realize these long-term goals,” Dr. Bock said.
Science
What to plant (and what to remove) in California’s new ‘Zone Zero’ fire-safety proposal
After years of heated debates among fire officials, scientists and local advocates, California’s Board of Forestry and Fire Protection released new proposed landscaping rules for fire-prone areas Friday that outline what residents can and can’t do within the first 5 feet of their homes.
Many of these proposed rules — designed to reduce the risk of a home burning down amid a wildfire — have wide support (or at least acceptance); however, the most contentious by far has been whether the state would allow healthy plants in the zone.
Many fire officials and safety advocates have essentially argued anything that can burn, will burn and have supported removing virtually anything capable of combustion from this zone within 5 feet of houses, dubbed “Zone Zero.” They point to the string of devastating urban wildfires in recent years as reason to move quickly.
Yet, researchers who study the array of benefits shade and extra foliage can bring to neighborhoods — and local advocates who are worried about the money and labor needed to comply with the regulations — have argued that this approach goes beyond what current science shows is effective. They have, instead, generally been in favor of allowing green, healthy plants within the zone.
The new draft regulations attempt to bridge the gap. They outline more stringent requirements to remove all plants in a new “Safety Zone” within a foot of the house and within a bigger buffer around potential vulnerabilities in a home’s wildfire armor, including windows that can shatter in extreme heat and wooden decks that can easily burst into flames. Everywhere else, the rules would allow residents to maintain some plants, although still with significant restrictions.
The rules generally do not require the removal of healthy trees — instead, they require giving these trees routine haircuts.
Once the state adopts a final version of the rules, homeowners would have three years to get their landscaping in order and up to five years for the bigger asks, including removing all vegetation from the Safety Zone and updating combustible fencing and sheds within 5 feet of the home. New constructions would have to comply immediately.
The rules only apply to areas with notable fire hazard, including urban areas that Cal Fire has determined have “very high” fire hazard and rural wildlands.
Officials with the Board will meet in Calabasas on Thursday from 1 p.m. to 7 p.m. to discuss the new proposal and hear from residents.
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Some L.A. residents are championing a proposed fire-safety rule, referred to as “Zone Zero,” requiring the clearance of flammable material within the first five feet of homes. Others are skeptical of its value.
Where is the Safety Zone?
The proposed Safety Zone with stricter requirements to remove all vegetation would extend 1 foot from the exterior walls of a house.
In a few areas with heightened vulnerabilities to wildfires, it extends further.
The Safety Zone covers any land under the overhang of roofs. If the overhang extends 3 feet, so does the Safety Zone in that area. It also extends 2 feet out from any windows, doors and vents, as well as 5 feet out from attached decks.
What plants would be allowed in the Safety Zone?
Generally, nothing that can burn can sit in the Safety Zone. This includes mulch, green grass, bushes and flowers.
What plants would be allowed in the rest of Zone Zero?
Homeowners can keep grasses (and other ground-covers, like moss) in this area, as long as it’s trimmed down to no taller than 3 inches.
The rules also allow small plants — from begonias to succulents — up to 18 inches tall as long as they are spaced out in groups. Residents can also keep spaced-out potted plants under this height, as long as they’re easily movable.
What about fences, trees and gates?
Any sheds or other outbuildings would need noncombustible exterior walls and roofs in Zone Zero — Safety Zone or not.
Residents would have to replace the first five feet of any combustible fencing or gates attached to their house with something made out of a noncombustible material, such as metal.
Trees generally would be allowed in Zone Zero. Homeowners would need to keep any branches one foot away from the walls, five feet above the roof and 10 feet from chimneys.
Residents would also have to remove any branches from the lower third of the tree (or up to 6 feet, whichever is shorter) to prevent fires on the ground from climbing into the canopy.
Some trees with trunks directly up against a house in this 1-foot buffer or under the roof’s overhang might need to go — since keeping branches away from the home could prove difficult (or impossible).
However, the board stressed it wants to avoid the removal of trees whenever feasible and encouraged homeowners to work with their local fire department’s inspectors to find case-by-case solutions.
What’s new and what’s not
Some of the rules discussed in Zone Zero are not new — they’ve been on the books for years, classified as requirements for Zone One, extending 30 feet from the home with generally less strict rules, and Zone Two, extending 100 feet from the house with the least strict rules.
For example, homeowners are already required to remove any dead or dying grasses, plants and trees. They also have to remove leaves, twigs and needles from gutters, and they already cannot keep exposed firewood in piles next to their house.
Residents are also already required to keep grasses shorter than 4 inches; Zone Zero lowers this by an inch.
Science
Video: Rescuers Mount a Likely Final Push to Save a Stranded Whale
new video loaded: Rescuers Mount a Likely Final Push to Save a Stranded Whale
By Jorge Mitssunaga
April 17, 2026
Science
1,200% jump in kratom-related calls to poison control centers in last decade, analysis shows
Over the last decade, poison control centers around the country have received tens of thousands of calls from consumers of kratom products reporting adverse and life-threatening health effects, with researchers saying reports in 2025 reached a new level. California’s poison center is reporting similar findings.
Last month, researchers analyzed information from the National Poison Data System and found that between 2015 and 2025, poison control centers across the nation received 14,449 calls related to kratom. More than 23% of those calls, or 3,434, were made last year, according to a published report in the Centers for Disease Control and Prevention. That represents a more than 1,200% increase from 2015, when only 258 calls were reported.
Officers gather illegally grown kratom plants in 2019 in Phang Nha province, Thailand. The country decriminalized the possession and sale of kratom in 2021.
(Associated Press)
Kratom is derived from the leaves of Mitragyna speciosa, a tree native to Southeast Asia. It has a long history of being used for chronic pain or to boost energy and in the U.S., research points to Americans also using it to alleviate anxiety. In low doses, kratom appears to act as a stimulant but in high doses, it can have effects more like opioids.
But in the last few years, a synthetic form of kratom refined for its psychoactive compound, 7-hydroxymitragynine or 7-OH, has entered the market that is highly concentrated and not clearly labeled, leading to confusion and problems for consumers. The synthetic form gaining momentum in the market is sparking concern among public health officials because of its ability to bind to opioid receptors in the body, causing it to have a higher potential for abuse.
Los Angeles County leaders, meanwhile, have grappled with differentiating the two and regulating the products that come in the form of powder, capsules and drinks and have been linked to six county deaths. Sales of kratom and 7-OH products were banned in the county in November.
In reviewing the data, which did not differentiate whether callers had consumed natural or synthetic kratom, researchers set out to understand the effect of what they believe is a “rapidly evolving kratom market,” and highlight the role poison centers can play as an early warning surveillance system to detect new trends.
National Poison Data System findings
The data showed that over the last 10 years, 62% of the kratom-related calls to poison control centers were from people who said they consumed the drug by itself, and the other 38% were from people who combined it with another substance or substances.
Those who consumed kratom with another substance combined it most frequently with one or a combination of the following: alcohol, opioids, benzodiazepines (like Xanax or Valium), cannabis and cannabinoids, stimulants and antidepressants.
The data also broke down hospitalizations related to kratom — adults who took it alone or in combination and experienced “adverse” health effects; and adults who took it alone or in combination and experienced more serious “moderate” or “major” health effects, including death.
Kratom powder products are displayed in a smoke shop in Los Angeles in 2024.
(Michael Blackshire/Los Angeles Times)
Hospitalizations for adults who had consumed kratom alone and experienced adverse effects increased from 43 in 2015 to 538 in 2025. For those who took it in combination and were hospitalized with an adverse health effect, the total jumped from 40 in 2015 to 549 last year.
The numbers were even higher for hospitalizations where the health effects were more serious or fatal.
In 2015, there were 76 reports of people being hospitalized after taking kratom alone and experiencing a serious health effect or dying. By last year, that number had climbed to 919. The reports of serious health effects, including death, for those who took kratom in combination with another substance grew from 51 in 2015 to 725 last year.
The research does not break down kratom-related deaths by year but states that there were 233 deaths over the 10-year study period, or just over 3% of all 7,287 serious medical outcomes. Of the total number of kratom-related deaths, 184 cases involved the consumption of multiple substances.
What California’s poison control system found in its state data
The California Poison Control System is currently reviewing its data concerning kratom-related calls but an initial analysis shows parallels to the national report, said Rais Vohra, medical director of the state poison control system.
“We have about 10% of the national population and about 10% of the national call volume with poison control,” Vohra said. “And so, not surprisingly, we were able to identify over 900 cases of calls related to kratom in that same period.”
Local researchers are still deciphering the state data but they too have found that kratom-related calls are climbing.
“It’s accelerating, which I think is one of the main points of the [published] report,” Vohra said.
A majority of calls received by poison control come from healthcare facilities where “presumably someone has a problem … severe enough to warrant calling 911 or going to the emergency room, and that’s when our agency gets involved,” Vohra said.
Kait Brown, clinical managing director for America’s Poison Control Centers, said the fact that kratom and 7-OH are federally unregulated products sold online, in gas stations and smoke shops gives people across the country easy access.
And while kratom enthusiasts maintain that it has been used in its natural form for hundreds of years, “there are new formulations that are a little bit different than how people have used it, at least historically,” said William Eggleston, a pharmacist and the assistant clinical director of the Upstate New York Poison Center in Syracuse.
People are no longer consuming kratom only as a powder or capsule but also in the form of an energy shot or extract; it’s similar for synthetic, more concentrated 7-OH products.
When regional poison centers compare their findings and experiences with the analysis of calls in the National Poison Data System, Eggleston said, “undeniably there is an increase in calls related to kratom.”
“But when you put it in the bigger perspective of all the calls … this is still a very small percentage of what we’re dealing with on a day to day basis,” he said.
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