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Louis Pasteur’s Relentless Hunt for Germs Floating in the Air

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Louis Pasteur’s Relentless Hunt for Germs Floating in the Air

Louis Pasteur was at his most comfortable when working in his Paris laboratory. It was there that he had some of his greatest scientific triumphs, including experiments that helped confirm germs can cause disease. “Everything gets complicated away from the laboratory,” he once complained to a friend.

But in 1860, years before he became famous for developing vaccines and heating milk to kill pathogens, Pasteur ventured to the top of a glacier, on a remarkable quest for invisible life.

He and a guide began at the base of Mont Blanc in the Alps, hiking through dark stands of pines. Behind them, a mule carried baskets of long‑necked glass chambers that sloshed with broth. They ascended a steep trail until they reached Mer de Glace, the sea of ice.

The wind blew briskly over the glacier, and the vale echoed with the sound of frozen boulders crashing down the slopes. Pasteur struggled to make out the path in the glare of sunlight bouncing off the ice.

When the scientist reached an altitude of 2,000 meters, he finally stopped. He removed one of the glass chambers from the mule’s pack and raised it over his head. With his free hand, he grabbed a pair of tongs and used them to snap off the end of the neck. The cold air rushed inside the container.

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The sight of Pasteur holding a globe of broth over his head would have baffled other travelers visiting Mer de Glace that day. If they had asked him what he was doing, his answer might have seemed mad. Pasteur was on a hunt, he later wrote, for “the floating germs of the air.”

Now, 165 years later, scientists around the world hunt for floating germs. Some study how coronaviruses wafting through buses and restaurants spread Covid. Spores of fungi can travel thousands of miles, infecting people and plants. Oceans deliver microbes into the air with every crashing wave. Even clouds, scientists now recognize, are alive with microbes.

The sky’s ecosystem is known as the aerobiome. In Pasteur’s day, it had no name. The very idea of living things drifting through the air was too strange to imagine.

But Pasteur began to wonder about the possibility of airborne life when he was a little-known chemist teaching at the University of Lille in France. There, the father of one his students approached him for help. The man owned a distillery where he used yeast to turn beet juice into alcohol. But the juice had inexplicably turned rancid.

Inspecting the liquid under a microscope, Pasteur discovered dark rods — bacteria rather than yeast — in the sour vats. The discovery helped him work out a theory of fermentation: Microorganisms absorbed nutrients and then produced new compounds. Depending on the species, they could turn butter rancid or grape juice into wine.

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The discovery won Pasteur a prestigious new post in Paris. In his account of the discovery, Pasteur suggested in passing that the bacteria might have floated through the air and settled into the vats. That notion earned him an angry letter from Félix‑Archimède Pouchet, one of France’s leading naturalists.

Pouchet informed Pasteur that the microorganisms Pasteur discovered had not dropped into the vats from the air. Instead, the beet juice had spontaneously generated them. “Spontaneous generation is the production of a new organized being that lacks parents and all of whose primordial elements have been drawn from ambient matter,” Pouchet had written earlier.

Pasteur coolly replied that Pouchet’s spontaneous generation experiments were fatally flawed. The conflict between Pasteur and Pouchet prompted the French Academy of Sciences to announce a contest for the best study addressing whether spontaneous generation was real or not. What started as a private spat had turned into a public spectacle. Pasteur and Pouchet both signed up to compete for the prize of 2,500 francs.

The public eagerly followed the competition, struggling to imagine either view of life. Spontaneous generation had the whiff of blasphemy: If life could spring into existence, it did not require divine intervention. But Pasteur’s claim that the atmosphere teemed with germs also strained the 19th‑century mind. A French journalist informed Pasteur that he was going to lose the contest. “The world into which you wish to take us is really too fantastic,” he said.

To prove that his world was real, Pasteur set out to pluck germs from the air. Working with glassblowers, he created flasks with narrow openings that stretched for several inches. He filled them with sterile broth and waited to see if anything would grow inside. If the necks were pointed straight up, the broth often turned cloudy with microorganisms. But if he sloped the necks so that the openings pointed down, the broth stayed clear. Pasteur argued that germs in the air could drift down into the flasks, but could not propel themselves up a rising path.

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When Pouchet heard about Pasteur’s experiments, he sneered. Did Pasteur really believe that every germ in decaying organic matter came from the air? If that were true, every cubic millimeter of air would have been packed with more germs than all the people on Earth. “The air in which we live would almost have the density of iron,” Pouchet said.

Pasteur responded by changing his hypothesis. Germs were not everywhere, he said. Instead, they drifted in clouds that were more common in some places than others.

To prove his claim, Pasteur took his straight-necked flasks out of his lab and began collecting germs. In the courtyard of the Paris Observatory, all 11 of his flasks turned cloudy with multiplying germs. But when he traveled to the countryside and ran his experiment again, more of his flasks stayed sterile. The farther Pasteur got from human settlements, the sparser airborne life became. To put that idea to an extreme test, Pasteur decided to climb Mer de Glace.

His first foray to the glacier ended in failure. After holding up a flask, he tried using the flame from a lamp to seal its neck shut, but the glare of the sun made the flame invisible. As Pasteur fumbled with the lamp, he worried that he might be contaminating the broth with germs he carried on his skin or his tools. He gave up and trudged to a tiny mountain lodge for the night.

He left his flasks open as he slept. In the morning they were rife with microorganisms. Pasteur concluded that the lodge was packed with airborne germs that travelers had brought from around the world.

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Later that day, Pasteur modified his lamp so that the flame would burn bright enough for him to see it under the glacier-reflected sun. When he climbed back up Mer de Glace, the experiment worked flawlessly. Only one of the flasks turned cloudy with germs. The other 19 remained sterile.

In November 1860, Pasteur arrived at the Academy of Sciences in Paris with the 73 flasks he had used on his travels. He entered the domed auditorium, walked up to the table where the prize committee sat, and laid out the flasks. The judges peered at the broth as Pasteur described his evidence, saying it gave “indubitable proof” of floating germs in inhabited places.

Pouchet refused to accept the evidence, but nevertheless withdrew from the contest. Pasteur was awarded the prize.

Still, the two continued to spar. The rivalry remained so intense that the Academy set up a new commission to evaluate their latest experiments. Pouchet dragged out the proceedings, demanding more time for his research.

Pasteur decided to seize public opinion and put on a spectacle. On the evening of April 7, 1864, in an amphitheater filled with Parisian elites, Pasteur stood surrounded by lab equipment and a lamp to project images on a screen. He told the audience it would not leave the soiree without recognizing that the air was rife with invisible germs. “We can’t see them now, for the same reason that, in broad daylight, we can’t see the stars,” he said.

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At Pasteur’s command, the lights went out, save for a cone of light that revealed floating motes of dust. Pasteur asked the audience to picture a rain of dust falling on every surface in the amphitheater. That dust, he said, was alive.

Pasteur then used a pump to drive air through a sterile piece of cotton. After soaking the cotton in water, he put a drop under a microscope. He projected its image on a screen for the audience to see. Alongside soot and bits of plaster, they could make out squirming corpuscles. “These, gentlemen, are the germs of microscopic beings,” Pasteur said.

Germs were everywhere in the air, he said — kicked up in dust, taking flights of unknown distances and then settling back to the ground, where they worked their magic of fermentation. Germs broke down “everything on the surface of this globe which once had life, in the general economy of creation,” Pasteur said.

“This role is immense, marvelous, positively moving,” he added.

The lecture ended with a standing ovation. Pasteur’s hunt for floating germs elevated him to the highest ranks of French science.

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By the time he died 31 years later, Pasteur had made so many world-changing discoveries that his many eulogies and obituaries did not mention his trip to Mer de Glace.

But scientists today recognize that Pasteur got the first glimpse of a world that they are only starting to understand. They now know that life infuses the atmosphere far more than he had imagined, all the way to the stratosphere. Our thriving aerobiome has led some scientists to argue that alien aerobiomes may float in the clouds of other planets. Ours is not the only world that seems too fantastic to believe.

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As mosquitoes go year-round in L.A., a promising fix hits a snag

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As mosquitoes go year-round in L.A., a promising fix hits a snag

Residents were supposed to get a respite from the ankle-nipping mosquitoes that fueled a recent surge in dengue fever in Los Angeles County.

Typically, the invasive mosquitoes — called Aedes aegypti — essentially disappear from winter until early May in the region.

Instead, complaints to local agencies tasked with controlling the pests spiked recently.

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“We have not seen them go away altogether like they have in previous years,” said Susanne Kluh, general manager for the Greater Los Angeles County Vector Control District.

Their unusual presence adds to the urgency of work going on in a 40-foot shipping container tucked away in Pacoima. It’s about to transform into a bustling nursery for tens of thousands of mosquitoes.

This May, the district is set for the third year in a row to release legions of sterilized male mosquitoes — which don’t bite — into parts of Sunland-Tujunga.

The last two years were promising, with the female population in two treated neighborhoods plunging by an average of more than 80%.

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Yet business owners have signaled they’re not willing to pay to expand it.

That’s thrown uncertainty into officials’ goal of eventually bringing the approach to their whole service area, spanning 36 cities and unincorporated communities.

Steve Vetrone, assistant general manager at the Greater L.A. vector district.

Steve Vetrone, assistant general manager at the Greater L.A. district.

(Ronaldo Bolanos / Los Angeles Times)

“Unfortunately, that’s going to be a rather expensive endeavor,” said Steve Vetrone, an assistant general manager for the district. “I can tell you right now that’s not something that we can do with our current operating budget.”

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A need, an ask and a disappointing answer

Aedes aegypti are a new-ish local fixture. Native to Africa, the black-and-white striped mosquitoes were first detected in California in 2013 and landed in L.A. County the following year.

“Despite our best efforts, they’ve been able to just outpace us, and they’re now in every city and community within our district,” and all of Southern California, Vetrone said. In fact, the low-flying, day-biting mosquitoes are present in nearly half of California’s counties, including Shasta in the far north.

Desperate to find a solution, many are trying the so-called sterile insect technique — including vector control districts serving Orange and San Bernardino counties, as well as the San Gabriel Valley — and “we kind of all hope that this is going to be our silver bullet,” Kluh said.

The idea is fairly simple: unleash sterile males so that they far outnumber wild ones — say, 10 to 1 or even 100 to 1. The goal is for the altered males to mate with females, producing eggs that don’t hatch.

Kluh’s district uses X-rays to sterilize males but there are other methods, such as using genetically modified insects or ones infected with bacteria.

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White mesh boxes holding mosquitoes on shelves inside a shipping container.

Female mosquitoes are fed different types of blood — pig and cow — to see which leads to the most eggs.

(Ronaldo Bolanos / Los Angeles Times)

The technique, while promising, requires time and money.

In California, property owners foot the bill for local mosquito (and other pest) control, with some paying an annual fee called a benefit assessment.

Levying a new fee requires approval from home, apartment and business owners, in accordance with Proposition 218.

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To unleash sterile male mosquitoes in a broader swath of the Greater L.A. district, officials are seeking up to $20 a year per single family home. That would be on top of $18.97 that homeowners now pay for the agency’s services.

Last April, the district sent out 50,000 sample ballots to property owners, asking if they’d support the increase.

Only 47% of those returned were in favor.

“Data showed that single family homeowners were pretty supportive, but fewer business owners with larger parcels and potentially higher dues did not see the benefit in the additional expense,” Kluh said in an email.

Business owners might not live in the area, but their vote — if their property spans several acres — is weighted more heavily.

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Times readers, commenting on a story from last year about the proposal, responded favorably.

“I hate mosquitos because they love me so much,” one reader said. “I would happily spend $20 to reduce their populations! I probably spend more [than] that on repellent.”

Officials haven’t given up, and plan to send out another round of sample ballots next year.

Kluh already has talking points for businesses in her back pocket: Restaurant owners should have an interest in making outdoor dining more pleasant, while apartment owners could lose revenue if their renters are sickened by an outbreak of Zika, chikungunya or yellow fever — all diseases transmitted by Aedes aegypti, she said.

Making mosquitoes that can’t reproduce

On a recent tour of the Pacoima insectary, Nicolas Tremblay, a senior vector ecologist with the district, whipped out a small container filled with a handful of what looked like vitamins.

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But the clear pill cases were filled with about 6,500 mosquito eggs and bovine liver powder.

Nicolas Tremblay, senior vector ecologist, places tape on water-filled trays in the Pacoima insectary.

Nicolas Tremblay, senior vector ecologist, tapes trays to indicate pill capsules filled with mosquito eggs were placed in water.

(Ronaldo Bolanos / Los Angeles Times)

The pills are dropped into trays of water, where the eggs hatch and the larvae feed on the powder. It takes about nine days to go from egg to buzzing adult.

The males are then chauffeured to Garden Grove, where they’re zapped with X-rays. Then they’re driven back and set free the next day.

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“It’s crazier around August, September, is when we’ll probably reach our peak production” of up to 72,000 mosquitoes a week, he said. “All these [trays] would be full of water and mosquitoes.”

In 2024, the district launched its pilot, releasing nearly 600,000 sterilized males in two Sunland-Tujunga neighborhoods over about five months.

The population of Aedes aegypti females dropped by an average of 82% compared with a control area.

The stakes became clear that year, when California reported 18 locally acquired dengue cases — a sharp rise from the first-ever cases confirmed the year before.

Last year, the pilot saw similar success, though there was also a natural drop in activity districtwide.

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On the recent visit to the insectary, several hundred mosquitoes flew around in white mesh cages, serving as participants in a study to see which blood they prefer — pig or cow.

“We haven’t completed the trials yet, but it seems like they didn’t care,” he said.

One thing scientists already know: Aedes aegypti love biting people.

A highly adaptive foe

The invasive mosquitoes can lay their eggs in tiny amounts of water. A bottle cap or crease in a potato chip bag is fair game.

What’s more, mosquitoes in the Greater L.A. district are resistant to a lot of pesticides.

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Now, there might be a new concern. Typically, the invasive mosquitoes go into a type of hibernation every year.

Kluh said it appeared that they may have mutated in a way that allows them to stay active through the winter.

A warming climate has already expanded their season and allowed them to move into formerly inhospitable regions.

Releasing sterilized males involves no pesticides, and also leverages the insect’s biology: Males in lust are adept at finding females.

Many residents are thrilled by the promising tool, but others bristle at the idea of manipulating nature.

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“There’s folks that are in favor and then there are folks that are just absolutely opposed because it’s like, ‘You’re playing God,’” Vetrone said.

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Record Heat Meets a Major Snow Drought Across the West

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Record Heat Meets a Major Snow Drought Across the West

At this point in a typical year, as the seasons officially turn from winter to spring, snowpack would still be accumulating across the Mountain West.

But this winter wasn’t typical, even before a heat wave this past week. It was the warmest on record for six Western states. Snow cover is the lowest level on record for the Colorado River Basin, and across much of the rest of the West, there are record or near-record low amounts of snow.

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That alone would create a challenging year for water managers, who rely on slow and steady snowmelt to feed streams, rivers and reservoirs and meet spring and summer demand for irrigation and drinking water. While rainfall runs off quickly and can more readily evaporate from soil, snowpack serves as a valuable and lasting source of moisture and accounts for a majority of water supplies across the region, as much as 80 percent in some areas.

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Current snowpack compared to historical averages

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The intense heat wave threatens to make water management all the more challenging.

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Much of the thin snowpack was already “ready to melt” before the heat set in, said Jon Meyer, assistant state climatologist at the Utah Climate Center. “This is the nail in the coffin.”

It’s unusual to see the whole West like this, said Leanne Lestak, an associate senior scientist at the University of Colorado Boulder who specializes in mapping snow and how much water it holds.

In early March, Ms. Lestak and her team found that vast majority of the Western United States had less than two-thirds of the amount of snow typical for this time of year, with few exceptions. In Arizona and parts of Nevada, New Mexico and Oregon, snowpack was less than a quarter of what it would usually be.

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“The situation is pretty dire,” Dr. Meyer said.

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The Cottonwood fire in Dawson County, Neb., on March 13. Nebraska State Patrol via AP

The heat wave is also increasing the already-elevated fire risk across some drought-stricken areas. In Nebraska, drought set the stage for the largest wildfire in state history, which broke out last week and has not yet been contained.

The conditions that led to this year’s low snowpack are unusual, too. Snow droughts often develop from dry weather patterns that starve the West of any significant precipitation during the winter, said Dan McEvoy, a climatologist at the Desert Research Institute and Western Regional Climate Center.

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But in many places, it wasn’t necessarily a dry year, he said. Instead, temperatures have been so warm that precipitation has fallen as rain, rather than snow, even at higher elevations.

Many of the mountaintops could still see some more snowfall. But as Cody Moser, a hydrologist with the Colorado Basin River Forecast Center in Salt Lake City, looks ahead to predicting how the spring will go, he doesn’t foresee any significant change in weather patterns. Now he’s expecting peak snowmelt flows to occur earlier than ever recorded in many locations, he said this week.

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“I think it’s highly likely we’ve seen peak snowpack,” Mr. Moser said.

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Snowpack feeding the Colorado River reaches historic lows

Source: USDA National Water and Climate Center

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Even after a winter that was the warmest on record for Arizona, New Mexico, Colorado, Utah, Wyoming and Oregon, the heat that set in across much of the West this past week was extreme. Meteorologists said they were expecting to set record highs for the month of March in many locations, and the earliest arrivals of 100-degree temperatures in records that go back more than a century.

Across the Colorado River Basin, even at elevations as high as 10,000 feet, temperatures were forecast to surge into the 50s and 60s Fahrenheit on Friday and Saturday, Mr. Moser said, some 15 to 20 degrees warmer than average.

Relatively light winds and dry air over the region could limit snowmelt to some degree, he said, but the warmth and sunshine may prevent some moisture from ever reaching stream beds, said John Fleck, a water policy expert at the University of New Mexico.

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“A lot of it is going to evaporate off before it even has a chance to hit the stream,” Mr. Fleck said.

This heat wave is so extreme that it would only be expected to occur once about every 500 years in the current climate, according to World Weather Attribution, a group of scientists who study links between extreme weather events and climate change.

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“These temperatures are completely off the scale for March, and our data shows that they would be virtually impossible in a world without human-caused climate change,” said Ben Clarke, a research associate in extreme weather and climate change at Imperial College London.

Ski trails in Park City, Utah, in February. Mario Tama/Getty Images

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In places like the Colorado Front Range, home to the majority of that state’s population, snowpack serves as the largest source of water. For the utility Denver Water, snowpack usually contains significantly more water than its largest surface reservoir, said Taylor Winchell, the agency’s climate adaptation program lead.

Denver Water has enough supply to handle a low-water year, but the snowpack conditions are creating “very high levels of concern,” Mr. Winchell said. The Denver Water Board is poised to officially declare Stage One drought restrictions, asking residents to significantly reduce their outdoor watering. If the snow drought were to repeat for multiple years, the problem could compound and worsen, he said.

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The snow drought occurs at a critical time for the larger Colorado River Basin. An agreement among the basin’s seven states over how to divide its water expired at the end of last year, and negotiations to develop a new water plan fell apart last month. (The states are also obligated to share a small portion of the water with Mexico.)

The snow drought is complicating that work. Snowpack from the river’s Upper Basin, across mountains of Colorado, Utah, New Mexico and Wyoming, accounts for a majority of the river’s natural flow each year. Declining spring precipitation and rising temperatures have caused the Colorado’s flow to decrease by nearly 20 percent over the past quarter century.

Recent forecasts estimated that inflows to Lake Powell, a key reservoir that straddles the Utah-Arizona border, will be the third-smallest on record. The lake’s surface could drop to a critical level for hydroelectric power production by the end of this year, affecting a power grid that serves seven states.

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Officials at the Bureau of Reclamation, the federal agency that oversees the Colorado River and its reservoirs, declined to be interviewed but said in a statement they were monitoring hydrologic conditions to guide decisions about how to manage the Colorado River system.

Mr. Fleck said a crisis without precedent could be brewing. While a drought that hit the basin in 2002 was worse, it was relatively more manageable than what the West now faces: “We’re having one of the worst years in many decades, but with no cushion of reservoir storage to fall back on to bail us out.”

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New report on L.A. post-fire beach contamination finds something unexpected: good news

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New report on L.A. post-fire beach contamination finds something unexpected: good news

Researchers investigating the long-term effects of the 2025 firestorms on L.A.’s beaches have found that rarest of things: good news.

In the year following the Palisades and Eaton fires, levels of harmful metals like lead in coastal sand and seawater have remained far below California’s limits for safe drinking water and the U.S. Environmental Protection Agency’s safety thresholds for aquatic life.

“We’re not seeing any evidence for harm in the ecosystem or harm for human health,” said Noelle Held, a University of Southern California marine biogeochemist and principal investigator for the CLEAN Waters project, which is measuring post-fire water quality.

The Palisades and Eaton fires burned more than 40,000 acres and destroyed at least 12,000 buildings, blanketing the ocean in ash for up to 100 miles offshore. Heavy rains a few weeks later washed the charred remnants of plastics, batteries, cars, chemicals and other potentially toxic material into the sea and up onto beaches via the region’s massive network of storm drains and concrete-lined rivers.

Initial testing by the nonprofit environmental group Heal the Bay in the weeks after the fires documented a spike in lead, mercury and other heavy metals in coastal waters. Concentrations of beryllium, copper, chromium, nickel and lead in particular were significantly above established safety thresholds for marine life, prompting fears for the long-term health of fish, marine mammals and the marine food chain.

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For their most recent study, Held’s team analyzed seawater samples collected along multiple locations on five different dates between Feb. 10 and Oct. 17 in 2025, along with sand collected in August.

Seawater lead concentrations were highest in the month after the fire and in October, when the season’s first major rain had just washed months’ worth of urban pollution into the ocean.

Even at their peak, lead levels barely surpassed 1 microgram per liter — well below the U.S. Environmental Protection Agency’s aquatic life safety threshold of 8.1 micrograms per liter.

While levels of iron, manganese and cobalt were higher in sampling locations near the Palisades burn scar than they were in other areas, even there they remain well below concentrations that could pose harm to human or marine life.

For beach sand collected in August, lead levels never topped 14 parts per million at any location, significantly below both the current California residential soil standard of 80 parts per million and the stricter 55 parts per million standard proposed by environmental health researchers.

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“This isn’t something we would flag if we were testing your soil in your yard,” Held said.

The recent findings are consistent with water quality tests the State Water Resources Control Board conducted earlier in 2025. A board spokesperson said those found both higher relative concentrations of metals closest to the burn scars and no overall evidence that post-fire pollution poses an ongoing threat to human health.

Yet the need for continued testing remains. Officials struggled to answer questions about post-fire beach safety in part because of a lack of historical data on pollution levels, a pitfall researchers would like to forestall before another disaster arrives.

Future rainstorms could also continue to wash metals into Will Rogers Beach and the Rustic Creek outfall, both of which are near the Palisades burn scar, CLEAN Waters warned.

“Post-fire impacts can change over time, depending on rainfalls, runoffs and sediment movements,” said Eugenia Ermacora, manager of the nonprofit Surfrider Foundation’s L.A. chapter, which has partnered with Held’s team to collect samples. “It’s not just about the fires, but it’s about urbanization and how much our city needs to continue the work of doing testing in the water.”

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