California health officials announced Wednesday that another batch of raw milk from Fresno-based Raw Farm has tested positive for bird flu virus, as dairy farms throughout the state continue to struggle with a widening number of outbreaks.

For the second time in roughly the span of a week, Santa Clara County Department of Public Health officials tested store-bought raw milk and found evidence of the virus. The sample was collected and tested on Tuesday.

Raw Farm is voluntarily recalling a batch of “cream top” whole milk half-gallon-size products, lot No. 20231119, expiration date Dec. 7.

According to Mark McAfee, the owner of Raw Farm, the state’s Department of Food and Agriculture has requested that he “hold delivery of further products” until Friday.

On Wednesday, state agriculture officials visited his farms in Fresno and Hanford, as well as his creamery in Fowler, and tested finished and unfinished products, as well as his trucks, bulk tanks and bottling facility.

He said the investigation was carried out with a thoroughness “like never before.”

There have been no reported illnesses associated with either this second recall or the first. The first recall also involved Raw Farm and was limited to a batch that was bottled on Nov. 9.

State and federal health officials say the H5N1 bird flu virus poses a low risk to the public. However, they have urged people not to drink raw, unpasteurized milk.

In addition, every waste site tested in California by WastewaterScan — an infectious-disease monitoring network led by researchers at Stanford and Emory University, with lab testing partner Verily, Alphabet Inc.’s life sciences organization — has tested positive for bird flu in the last two weeks.

The Los Angeles County Department of Public Health issued a statement late Wednesday expanding the list of stores that may have sold bird-flu-contaminated raw milk.

They include:

• Back Door Bakery, 8349 Foothill Blvd., Sunland, CA 91040
• Bristol Farms, 7880 Sunset Blvd., Los Angeles, CA 90046
• Eataly, 10250 Santa Monica Blvd., Los Angeles, CA 90067
• Erewhon Market, 475 S. Lake Ave., Pasadena, CA 91101
• Erewhon Market, 26767 Agoura Road, Calabasas, CA 91302
• Erewhon Market, 4121 Santa Monica Blvd., Los Angeles, CA 90029
• Erewhon Market, 7660 Beverly Blvd., Los Angeles, CA 90036
• Lassen’s Natural Foods, 1631 W. Sunset Blvd., Los Angeles, CA 90026
• Lassen’s Natural Foods, 2080 Hillhurst Ave., Los Angeles, CA 90027
• Lassen’s Natural Foods, 710 S. La Brea Ave., Los Angeles, CA 90036
• Lazy Acres, 1841 N. Western Ave., Los Angeles, CA 90027
• Lazy Acres, 2510 Pacific Coast Highway, Hermosa Beach, CA 90254
• Mothers Market & Kitchen, 6677 W. Santa Monica Blvd., Los Angeles, CA 90038
• Mothers Market & Kitchen, 2475 Cherry Ave., Signal Hill, CA 90755
• Sprouts Farmers Market, 1302 S. La Brea Ave., Los Angeles CA 90019
• Sprouts Farmers Market, 1751 Westwood Blvd., Los Angeles CA 90024
• Sprouts Farmers Market, 8985 Venice Blvd., Los Angeles CA 90034
• Sprouts Farmers Market, 915 N. La Brea Ave., Los Angeles, CA 90038
• Sprouts Farmers Market, 2245 Yosemite Drive, Eagle Rock, 90041
• Sprouts Farmers Market, 7925 Atlantic Ave., Cudahy CA, 90201
• Sprouts Farmers Market, 5660 Sepulveda Blvd., Culver City CA, 90230
• Sprouts Farmers Market, 12060 Lakewood Blvd., Downey, CA 90242
• Sprouts Farmers Market, 14411 Hawthorne Blvd., Lawndale, CA 90260
• Sprouts Farmers Market, 1515 Hawthorne Blvd., Redondo Beach, CA 90278
• Sprouts Farmers Market, 4230 Pacific Coast Highway, Torrance CA, 90505
• Sprouts Farmers Market, 15801 Whittier Blvd., Whittier, CA 90603
• Sprouts Farmers Market, 11522 Alondra Blvd., Norwalk, CA 90650
• Sprouts Farmers Market, 11900 South St., Cerritos, CA 90703
• Sprouts Farmers Market, 4253 Woodruff Ave., Lakewood, CA 90713
• Sprouts Farmers Market, 820 N. Western Ave., San Pedro, CA 90732
• Sprouts Farmers Market, 4600 E. Pacific Coast Highway, Long Beach, CA 90804
• Sprouts Farmers Market, 920 Foothill Blvd., La Cañada Flintridge, CA 91011
• Sprouts Farmers Market, 400 W. Huntington Drive, Monrovia, CA 91016
• Sprouts Farmers Market, 39 N. Rosemead Blvd., Pasadena, CA 91107
• Sprouts Farmers Market, 6607 Fallbrook Ave., West Hills, CA 91307
• Sprouts Farmers Market, 10821 N. Zelzah Ave., Granada Hills, CA 91344
• Sprouts Farmers Market, 24285 Magic Mountain Pkwy., Valencia, CA 91355
• Sprouts Farmers Market, 21821 Ventura Blvd., Woodland Hills, CA 91364
• Sprouts Farmers Market, 1011 N. San Fernando Blvd., Burbank, CA 91504
• Sprouts Farmers Market, 11315 Ventura Blvd., Studio City, CA 91604
• Sprouts Farmers Market, 835 W. Foothill Blvd., Claremont, CA 91711
• Sprouts Farmers Market, 655 S Grand Ave., Glendora, CA 91740
• Sprouts Farmers Market, 1375 Foothill Blvd., La Verne, CA 91750
• Sprouts Farmers Market, 239 S. Diamond Bar Blvd., Diamond Bar, CA 91765
• Sprouts Farmers Market, 2630 E. Workman Ave., West Covina, CA 91791
• Sprouts Farmers Market, 150 E Main St., Alhambra, CA 91801
• Vitamin City LB, 6247 E. Spring St., Long Beach, CA 90808
• Vitamin City, 642 W. Arrow Highway, San Dimas, CA 91773
• Whole Wheatery, 44264 10th W., Lancaster, CA 93534

Your heart is racing, your arms are tingling and your breathing is shallow. You’re having an anxiety attack. And you’re in a public place, to boot. A crowded restaurant, say, or at the office. Not a space where you can comfortably lay on the ground and do some deep breathing exercises to calm yourself.

What if there were a pill that would instead induce that kind of calm breathing for you? That scenario might be possible after a new scientific breakthrough.

Neuroscientists at the Salk Institute for Biological Studies in La Jolla have identified a brain pathway that instantly deflates anxiety. The new study, which published earlier this week in the scientific journal Nature Neuroscience, lays out how the aforementioned brain circuit regulates voluntary breathing — meaning conscious breathing as opposed to automatic breathing that happens without your having to think about it — allowing us to slow our breath and calm our mind.

The discovery opens up the potential for the creation of new drugs that would mimic the relaxed state common during breath work, meditation or yoga. Sung Han, senior author of the study, says he’d like to one day see a “yoga pill,” as he calls it, on the market to ease anxiety. It would likely be useful for the more than 40 million adults in the U.S, who, according to the National Alliance on Mental Illness, suffer from an anxiety disorder.

Han says the new discovery is a real scientific breakthrough.

“As a scientist, finding something never known before is always exciting,” he told the Los Angeles Times. “This top-down breathing circuit has been a longstanding question in the neuroscience field. It’s exciting to find the neural mechanism to explain how the slowing down of breathing can control negative emotions, like anxiety and fear.”

We’ve long known that we can control our breathing patterns to alter our state of mind — when we get stressed, we might take a deep, slow breath to feel calmer. But scientists didn’t understand how that worked — which parts of the brain were actually slowing our breath and why that activity makes us calmer. Now they know that there is a group of cells in the cortex, the higher part of the brain responsible for more conscious, complex thought, that send messages to the brain stem, which in turn sends information to the lungs. That’s the aforementioned “circuit.”

The discovery validates soothing behavioral practices such as yoga, mindfulness and even “box breathing” — the latter a technique that involves repeatedly breathing in, then holding your breath, for four-second counts in order to relieve stress — because it grounds these behavioral practices in science.

But the practical applications is what makes the Salk discovery so important, Han says.

“It can, potentially, create a whole new class of drugs that can more specifically target anxiety disorder,” he says.

These would differ from common anti-anxiety medications by more specifically targeting areas of the brain. Common anti-anxiety drugs like Xanax and Lexapro target multiple areas of the brain that control multiple brain processes and behaviors. It’s why these drugs don’t work for everyone in the same way and may create unwanted side effects. More precisely targeting an individual brain circuit makes a medication more effective and reduces potential side effects. And, in extreme cases, such a pill might be more efficient for targeting anxiety than doing breathing exercises.

“If you’re in panic, breathing techniques alone may not be sufficient to suppress anxiety,” Han says.

Han’s team is now trying to find the opposite circuit — a fast breathing circuit — that increases anxiety.

“To target the slow breathing circuit, we need to understand the opposite circuit, so we can avoid targeting it,” Han says. “To relieve the anxiety.”

While Han hopes his findings will lead to a “yoga pill,” that’s likely a long ways off. The research, and ensuing clinical trials, could take as much as 10 years, he says. And nothing is for certain.

“I cannot say that this discovery is directly connected to the discovery of the new medication,” Han says. “But I can say it’s a stepping stone. We now know the pathway. That’s exciting. That is the first step.”

TIPTON, Calif. —  It was a late fall morning and hundreds of cows — black and white splattered Holsteins and cappuccino-colored Jerseys — milled about a San Joaquin Valley dairy farm in the largest milk-producing state in the nation.

Nearby, workers herded some of the animals onto a rotating platform within the farm’s milking parlor and quickly attached pumping equipment. The machines buzzed and whirred as the cows were carried in a lazy arc to the parlor’s exit, where they were detached from milk hoses and sent on their way.

The scene seemed utterly unremarkable — except for the fact that five days earlier, the H5N1 bird flu virus that has ravaged California’s dairy herds for the last three months, had been confirmed on the farm. Although dozens of cows were sick, and their owner expected that number to climb, none of the farm’s workers wore personal protective equipment and vehicles from off site were let in and out with nary a hint of concern.

The farm was just one of more than 400 California operations that have been confirmed to have suffered outbreaks of H5N1, but interviews with Central Valley dairy farmers, dairy workers and a recent study by the U.S. Centers for Disease Control and Prevention suggests the virus may be more widespread in people than the CDC’s official numbers suggest. Although authorities have been urging dairy farms and workers to take precautions against spreading infection, there is little evidence their cautions are being heeded.

No expert will say that H5N1 bird flu is going to become the next global pandemic, and government health officers say the virus poses a low risk to the public. However, some experts warn that nearly all the conditions needed for the virus to develop a threatening mutation are now present in many dairy farms: Lax testing protocols; close, unprotected contact between humans and animals; a general failure to take the threat seriously enough; and the approach of human flu season.

Since this particular clade of H5N1 virus (2.3.4.4b) first appeared in North America at the end of 2021, it has infected more than 600 dairy herds across 15 states, hundreds of millions of wild and domesticated birds, and at least 48 mammal species — such as dolphins, seals, cats and dogs.

“We are in a soup of virus. I mean, there’s virus everywhere around us at this point,” said Dr. Marcela Uhart, a wildlife veterinarian with UC Davis’ One Health institute, who is based in Argentina, speaking at symposium held by the O’Neill Institute at Georgetown Law recently. (11/15) “This virus is circulating left and right in mammals and in birds, as far as we can tell, some of them are not showing any signs of disease.”

In October, The Times visited Tipton, a dairy town in Tulare County, and spoke with several residents, including Elodia Ibañez, who said the number of reported human cases in California — which in mid-October was 16, and is now 23 — sounded too low. She said her husband, a dairy worker, told her that two of his co-workers had red, swollen eyes, but they had continued working despite displaying bird flu symptoms.

“It’s an illness that they know the cows have, and many cows have died. But the boss cared about the cows, not the workers,” Ibañez told The Times. “They never told them they have to go to the doctor to get a check-up.”

People often continue to work because they feel they have no other choice, she said. “Even though they say there are laws that protect them, there are still a lot of people who are fearful … They’re scared of losing their jobs.”

Anthony, also a Tipton resident, said he would talk, but did not want to share his last name; he has family members who work in dairies, and he feared impacting their employment by speaking out.

“My dad and uncle have told me there’s a bunch of dairies that have had outbreaks,” Anthony said. He said his dad and uncle take steps to stay safe, but many workers likely are not reporting getting sick because they don’t want to get in trouble.

“Some of them are here not legally. They’re relying on that job, they don’t want to jeopardize that,” he said.

In early November, the CDC published a study that looked for H5N1 antibodies in the blood of dairy workers in Michigan and Colorado. The agency sampled blood from 115 people; eight — or 7% — had antibodies. Only three dairy workers in those two states — one in Colorado, two in Michigan — have been positively identified as having the disease.

It’s this blindness to — and ignorance of — the virus’ reach that has infectious disease and health experts concerned.

And as human flu season approaches, and infected wild birds continue their southward migration down the North American flyways — stopping to rest in lakes, ponds, farms and backyards throughout the United States — experts worry conditions are becomingly increasingly ripe for a large “spillover” event.

If a virus — whether it’s a bird flu, a human influenza virus or a coronavirus — is given the opportunity to spread within and between organisms, the virus will evolve, adapt and mutate. Sometimes these mutations have little effect on its ability to transmit between organisms or cause severe disease. But sometimes, they do.

Then there’s the concern that the bird flu virus will find another flu virus that’s circulating — a human, swine or even other bird flu — and swap genetic material with it, potentially creating a new “super flu” that can spread easily between people, make its hosts very sick, or carry immunity to the antiviral medications used to treat infected patients.

In the 1970s, when the understanding of flu viruses was still being developed, Robert Webster, a researcher at St. Jude’s Hospital in Memphis, Tenn., conducted an experiment at the U.S. Department of Agriculture’s Plum Island Research Station, off the coast of Long Island.

He put a human flu virus — H3N2 — into one pig, and a swine flu virus — H1N1 — into another. He then put the two pigs in a pen with four other pigs. Seven days later, he and his team detected reassorted viruses — H3N1 and H1N2 — in one of the other pigs.

The viruses had swapped genes and created new combinations.

Jersey cows drink water at sunrise.

(Tomas Ovalle / For The Times)

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The work of Webster and others led to the discovery that several of the nastier historical flu pandemics have been the result of this kind of reassortment. For instance, the 1918 flu outbreak — which killed approximately 50 million people worldwide — is believed to have been a recombined version of a bird and human flu.

It happened again in 2009, when a human and swine flu switched genes, unleashing the H1N1 swine flu outbreak that killed roughly 500,000 people.

Already there is evidence this virus is swapping genes. The birds currently traveling south from the Arctic are carrying a slightly different variant of H5N1 — called D1.1 or D1.2 — that has an altered take on the several of the flu’s viral segments.

It’s this strain that has put a Canadian teenager into critical condition at a British Columbia hospital. Health authorities don’t know where or how the child picked it up, except to note it wasn’t from cows or poultry. And they don’t know yet whether it has acquired the ability to move easily between people — although early and initial testing suggests it may have acquired some new and ominous traits, including changes that would make it easier to infect people.

Even so, as of this moment, there is no evidence that the H5N1 bird flu circulating among dairy cows and workers — known as B3.13 — has achieved the ability to move efficiently from human to human, or to cause severe illness. But with human flu season approaching — and the possibility that retail customers were drinking infected raw milk — the chances increase.

In an effort to spread awareness among dairy farms and potentially lure workers in for testing and decrease the chances for this virus to acquire mutations that could make it widespread and deadly in people, state health, agriculture and workers safety experts held a workshop at the Tulare Expo Center in late October, with help from the dairy trade group, Western United Dairies.

About 20 dairy workers and farmers sat through the two-hour session, which included English and Spanish presentations from the different state agenciess and dozens of slides

Dr. Erica Pan, California’s State Epidemiologist, told the audience her agency recommended personal protective equipment at work, and urged people to protect their eyes. She also told the audience to stay up to date on their recommended vaccines and refrain from consuming raw milk and undercooked beef, “especially ground beef.”

Eric Berg, Deputy Chief of Health and Research and Standards at CalOSHA said that farms under quarantine needed to establish restricted areas for infected animals. Workers, he said, should wear protective clothing, including coveralls, gloves and “and very important … eye protection and also respirators indoors.”

Eduardo Mondragon sat near the front of a conference room, nodding along as experts discussed safety protocols for dairy workers. As a manager of multiple dairy farms, Mondragon had watched the bird flu rip through the farms he oversaw in Tulare County and the cows he and his colleagues were tasked with caring for as they became sick.

Milk production fell as hundreds of cows became sick and about a dozen died, he said, but the dairy’s owner provided protective gear, including gloves and goggles, which workers were used to wearing daily.

At the dairy farm, Mondragon said they worked quickly when cows started getting sick over the summer to try and stop the spread. His boss sent him to the seminar in Tulare to learn more about the bird flu.

“For weeks, we never stopped,” he said, with many workers working weekend shifts to care for the cows. “We had a good handle on the flu, and because of that we didn’t suffer that many losses. Milk production yes, but animals, no.”

Mondragon knows that while his dairy farm saw the worst of the bird flu hit over the summer, none of the workers reported getting sick. He said friends in the industry in other countries were not yet affected like dairies in Tulare County.

After the seminar, Mondragon loaded up his white pickup truck with boxes of N95 masks, face shields and goggles to take back to his job site and share with the other workers.