Science
Louis Pasteur’s Relentless Hunt for Germs Floating in the Air
Louis Pasteur was at his most comfortable when working in his Paris laboratory. It was there that he had some of his greatest scientific triumphs, including experiments that helped confirm germs can cause disease. “Everything gets complicated away from the laboratory,” he once complained to a friend.
But in 1860, years before he became famous for developing vaccines and heating milk to kill pathogens, Pasteur ventured to the top of a glacier, on a remarkable quest for invisible life.
He and a guide began at the base of Mont Blanc in the Alps, hiking through dark stands of pines. Behind them, a mule carried baskets of long‑necked glass chambers that sloshed with broth. They ascended a steep trail until they reached Mer de Glace, the sea of ice.
The wind blew briskly over the glacier, and the vale echoed with the sound of frozen boulders crashing down the slopes. Pasteur struggled to make out the path in the glare of sunlight bouncing off the ice.
When the scientist reached an altitude of 2,000 meters, he finally stopped. He removed one of the glass chambers from the mule’s pack and raised it over his head. With his free hand, he grabbed a pair of tongs and used them to snap off the end of the neck. The cold air rushed inside the container.
The sight of Pasteur holding a globe of broth over his head would have baffled other travelers visiting Mer de Glace that day. If they had asked him what he was doing, his answer might have seemed mad. Pasteur was on a hunt, he later wrote, for “the floating germs of the air.”
Now, 165 years later, scientists around the world hunt for floating germs. Some study how coronaviruses wafting through buses and restaurants spread Covid. Spores of fungi can travel thousands of miles, infecting people and plants. Oceans deliver microbes into the air with every crashing wave. Even clouds, scientists now recognize, are alive with microbes.
The sky’s ecosystem is known as the aerobiome. In Pasteur’s day, it had no name. The very idea of living things drifting through the air was too strange to imagine.
But Pasteur began to wonder about the possibility of airborne life when he was a little-known chemist teaching at the University of Lille in France. There, the father of one his students approached him for help. The man owned a distillery where he used yeast to turn beet juice into alcohol. But the juice had inexplicably turned rancid.
Inspecting the liquid under a microscope, Pasteur discovered dark rods — bacteria rather than yeast — in the sour vats. The discovery helped him work out a theory of fermentation: Microorganisms absorbed nutrients and then produced new compounds. Depending on the species, they could turn butter rancid or grape juice into wine.
The discovery won Pasteur a prestigious new post in Paris. In his account of the discovery, Pasteur suggested in passing that the bacteria might have floated through the air and settled into the vats. That notion earned him an angry letter from Félix‑Archimède Pouchet, one of France’s leading naturalists.
Pouchet informed Pasteur that the microorganisms Pasteur discovered had not dropped into the vats from the air. Instead, the beet juice had spontaneously generated them. “Spontaneous generation is the production of a new organized being that lacks parents and all of whose primordial elements have been drawn from ambient matter,” Pouchet had written earlier.
Pasteur coolly replied that Pouchet’s spontaneous generation experiments were fatally flawed. The conflict between Pasteur and Pouchet prompted the French Academy of Sciences to announce a contest for the best study addressing whether spontaneous generation was real or not. What started as a private spat had turned into a public spectacle. Pasteur and Pouchet both signed up to compete for the prize of 2,500 francs.
The public eagerly followed the competition, struggling to imagine either view of life. Spontaneous generation had the whiff of blasphemy: If life could spring into existence, it did not require divine intervention. But Pasteur’s claim that the atmosphere teemed with germs also strained the 19th‑century mind. A French journalist informed Pasteur that he was going to lose the contest. “The world into which you wish to take us is really too fantastic,” he said.
To prove that his world was real, Pasteur set out to pluck germs from the air. Working with glassblowers, he created flasks with narrow openings that stretched for several inches. He filled them with sterile broth and waited to see if anything would grow inside. If the necks were pointed straight up, the broth often turned cloudy with microorganisms. But if he sloped the necks so that the openings pointed down, the broth stayed clear. Pasteur argued that germs in the air could drift down into the flasks, but could not propel themselves up a rising path.
When Pouchet heard about Pasteur’s experiments, he sneered. Did Pasteur really believe that every germ in decaying organic matter came from the air? If that were true, every cubic millimeter of air would have been packed with more germs than all the people on Earth. “The air in which we live would almost have the density of iron,” Pouchet said.
Pasteur responded by changing his hypothesis. Germs were not everywhere, he said. Instead, they drifted in clouds that were more common in some places than others.
To prove his claim, Pasteur took his straight-necked flasks out of his lab and began collecting germs. In the courtyard of the Paris Observatory, all 11 of his flasks turned cloudy with multiplying germs. But when he traveled to the countryside and ran his experiment again, more of his flasks stayed sterile. The farther Pasteur got from human settlements, the sparser airborne life became. To put that idea to an extreme test, Pasteur decided to climb Mer de Glace.
His first foray to the glacier ended in failure. After holding up a flask, he tried using the flame from a lamp to seal its neck shut, but the glare of the sun made the flame invisible. As Pasteur fumbled with the lamp, he worried that he might be contaminating the broth with germs he carried on his skin or his tools. He gave up and trudged to a tiny mountain lodge for the night.
He left his flasks open as he slept. In the morning they were rife with microorganisms. Pasteur concluded that the lodge was packed with airborne germs that travelers had brought from around the world.
Later that day, Pasteur modified his lamp so that the flame would burn bright enough for him to see it under the glacier-reflected sun. When he climbed back up Mer de Glace, the experiment worked flawlessly. Only one of the flasks turned cloudy with germs. The other 19 remained sterile.
In November 1860, Pasteur arrived at the Academy of Sciences in Paris with the 73 flasks he had used on his travels. He entered the domed auditorium, walked up to the table where the prize committee sat, and laid out the flasks. The judges peered at the broth as Pasteur described his evidence, saying it gave “indubitable proof” of floating germs in inhabited places.
Pouchet refused to accept the evidence, but nevertheless withdrew from the contest. Pasteur was awarded the prize.
Still, the two continued to spar. The rivalry remained so intense that the Academy set up a new commission to evaluate their latest experiments. Pouchet dragged out the proceedings, demanding more time for his research.
Pasteur decided to seize public opinion and put on a spectacle. On the evening of April 7, 1864, in an amphitheater filled with Parisian elites, Pasteur stood surrounded by lab equipment and a lamp to project images on a screen. He told the audience it would not leave the soiree without recognizing that the air was rife with invisible germs. “We can’t see them now, for the same reason that, in broad daylight, we can’t see the stars,” he said.
At Pasteur’s command, the lights went out, save for a cone of light that revealed floating motes of dust. Pasteur asked the audience to picture a rain of dust falling on every surface in the amphitheater. That dust, he said, was alive.
Pasteur then used a pump to drive air through a sterile piece of cotton. After soaking the cotton in water, he put a drop under a microscope. He projected its image on a screen for the audience to see. Alongside soot and bits of plaster, they could make out squirming corpuscles. “These, gentlemen, are the germs of microscopic beings,” Pasteur said.
Germs were everywhere in the air, he said — kicked up in dust, taking flights of unknown distances and then settling back to the ground, where they worked their magic of fermentation. Germs broke down “everything on the surface of this globe which once had life, in the general economy of creation,” Pasteur said.
“This role is immense, marvelous, positively moving,” he added.
The lecture ended with a standing ovation. Pasteur’s hunt for floating germs elevated him to the highest ranks of French science.
By the time he died 31 years later, Pasteur had made so many world-changing discoveries that his many eulogies and obituaries did not mention his trip to Mer de Glace.
But scientists today recognize that Pasteur got the first glimpse of a world that they are only starting to understand. They now know that life infuses the atmosphere far more than he had imagined, all the way to the stratosphere. Our thriving aerobiome has led some scientists to argue that alien aerobiomes may float in the clouds of other planets. Ours is not the only world that seems too fantastic to believe.
Science
Diablo Canyon clears last California permit hurdle to keep running
Central Coast Water authorities approved waste discharge permits for Diablo Canyon nuclear plant Thursday, making it nearly certain it will remain running through 2030, and potentially through 2045.
The Pacific Gas & Electric-owned plant was originally supposed to shut down in 2025, but lawmakers extended that deadline by five years in 2022, fearing power shortages if a plant that provides about 9 percent the state’s electricity were to shut off.
In December, Diablo Canyon received a key permit from the California Coastal Commission through an agreement that involved PG&E giving up about 12,000 acres of nearby land for conservation in exchange for the loss of marine life caused by the plant’s operations.
Today’s 6-0 vote by the Central Coast Regional Water Board approved PG&E’s plans to limit discharges of pollutants into the water and continue to run its “once-through cooling system.” The cooling technology flushes ocean water through the plant to absorb heat and discharges it, killing what the Coastal Commission estimated to be two billion fish each year.
The board also granted the plant a certification under the Clean Water Act, the last state regulatory hurdle the facility needed to clear before the federal Nuclear Regulatory Commission (NRC) is allowed to renew its permit through 2045.
The new regional water board permit made several changes since the last one was issued in 1990. One was a first-time limit on the chemical tributyltin-10, a toxic, internationally-banned compound added to paint to prevent organisms from growing on ship hulls.
Additional changes stemmed from a 2025 Supreme Court ruling that said if pollutant permits like this one impose specific water quality requirements, they must also specify how to meet them.
The plant’s biggest water quality impact is the heated water it discharges into the ocean, and that part of the permit remains unchanged. Radioactive waste from the plant is regulated not by the state but by the NRC.
California state law only allows the plant to remain open to 2030, but some lawmakers and regulators have already expressed interest in another extension given growing electricity demand and the plant’s role in providing carbon-free power to the grid.
Some board members raised concerns about granting a certification that would allow the NRC to reauthorize the plant’s permits through 2045.
“There’s every reason to think the California entities responsible for making the decision about continuing operation, namely the California [Independent System Operator] and the Energy Commission, all of them are sort of leaning toward continuing to operate this facility,” said boardmember Dominic Roques. “I’d like us to be consistent with state law at least, and imply that we are consistent with ending operation at five years.”
Other board members noted that regulators could revisit the permits in five years or sooner if state and federal laws changes, and the board ultimately approved the permit.
Science
Deadly bird flu found in California elephant seals for the first time
The H5N1 bird flu virus that devastated South American elephant seal populations has been confirmed in seals at California’s Año Nuevo State Park, researchers from UC Davis and UC Santa Cruz announced Wednesday.
The virus has ravaged wild, commercial and domestic animals across the globe and was found last week in seven weaned pups. The confirmation came from the U.S. Department of Agriculture’s National Veterinary Services Laboratory in Ames, Iowa.
“This is exceptionally rapid detection of an outbreak in free-ranging marine mammals,” said Professor Christine Johnson, director of the Institute for Pandemic Insights at UC Davis’ Weill School of Veterinary Medicine. “We have most likely identified the very first cases here because of coordinated teams that have been on high alert with active surveillance for this disease for some time.”
Since last week, when researchers began noticing neurological and respoiratory signs of the disease in some animals, 30 seals have died, said Roxanne Beltran, a professor of ecology and evolutionary biology at UC Santa Cruz. Twenty-nine were weaned pups and the other was an adult male. The team has so far confirmed the virus in only seven of the dead pups.
Infected animals often have tremors convulsions, seizures and muscle weakness, Johnson said.
Beltran said teams from UC Santa Cruz, UC Davis and California State Parks monitor the animals 260 days of the year, “including every day from December 15 to March 1” when the animals typically come ashore to breed, give birth and nurse.
The concerning behavior and deaths were first noticed Feb. 19.
“This is one of the most well-studied elephant seal colonies on the planet,” she said. “We know the seals so well that it’s very obvious to us when something is abnormal. And so my team was out that morning and we observed abnormal behaviors in seals and increased mortality that we had not seen the day before in those exact same locations. So we were very confident that we caught the beginning of this outbreak.”
In late 2022, the virus decimated southern elephant seal populations in South America and several sub-Antarctic Islands. At some colonies in Argentina, 97% of pups died, while on South Georgia Island, researchers reported a 47% decline in breeding females between 2022 and 2024. Researchers believe tens of thousands of animals died.
More than 30,000 sea lions in Peru and Chile died between 2022 and 2024. In Argentina, roughly 1,300 sea lions and fur seals perished.
At the time, researchers were not sure why northern Pacific populations were not infected, but suspected previous or milder strains of the virus conferred some immunity.
The virus is better known in the U.S. for sweeping through the nation’s dairy herds, where it infected dozens of dairy workers, millions of cows and thousands of wild, feral and domestic mammals. It’s also been found in wild birds and killed millions of commercial chickens, geese and ducks.
Two Americans have died from the virus since 2024, and 71 have been infected. The vast majority were dairy or commercial poultry workers. One death was that of a Louisiana man who had underlying conditions and was believed to have been exposed via backyard poultry or wild birds.
Scientists at UC Santa Cruz and UC Davis increased their surveillance of the elephant seals in Año Nuevo in recent years. The catastrophic effect of the disease prompted worry that it would spread to California elephant seals, said Beltran, whose lab leads UC Santa Cruz’s northern elephant seal research program at Año Nuevo.
Johnson, the UC Davis researcher, said the team has been working with stranding networks across the Pacific region for several years — sampling the tissue of birds, elephant seals and other marine mammals. They have not seen the virus in other California marine mammals. Two previous outbreaks of bird flu in U.S. marine mammals occurred in Maine in 2022 and Washington in 2023, affecting gray and harbor seals.
The virus in the animals has not yet been fully sequenced, so it’s unclear how the animals were exposed.
“We think the transmission is actually from dead and dying sea birds” living among the sea lions, Johnson said. “But we’ll certainly be investigating if there’s any mammal-to-mammal transmission.”
Genetic sequencing from southern elephant seal populations in Argentina suggested that version of the virus had acquired mutations that allowed it to pass between mammals.
The H5N1 virus was first detected in geese in China in 1996. Since then it has spread across the globe, reaching North America in 2021. The only continent where it has not been detected is Oceania.
Año Nuevo State Park, just north of Santa Cruz, is home to a colony of some 5,000 elephant seals during the winter breeding season. About 1,350 seals were on the beach when the outbreak began. Other large California colonies are located at Piedras Blancas and Point Reyes National Sea Shore. Most of those animals — roughly 900 — are weaned pups.
It’s “important to keep this in context. So far, avian influenza has affected only a small proportion of the weaned at this time, and there are still thousands of apparently healthy animals in the population,” Beltran said in a press conference.
Public access to the park has been closed and guided elephant seal tours canceled.
Health and wildlife officials urge beachgoers to keep a safe distance from wildlife and keep dogs leashed because the virus is contagious.
Science
When slowing down can save a life: Training L.A. law enforcement to understand autism
Kate Movius moved among a roomful of Los Angeles County sheriff’s deputies, passing out a pop trivia quiz and paper prism glasses.
She told them to put on the vision-distorting glasses, and to write with their nondominant hand. As they filled out the tests, Movius moved about the City of Industry classroom pounding abruptly on tables. Then came the cowbell. An aide flashed the overhead lights on and off at random. The goal was to help the deputies understand the feeling of sensory overwhelm, which many autistic people experience when incoming stimulation exceeds their capacity to process.
“So what can you do to assist somebody, or de-escalate somebody, or get information from someone who suffers from a sensory disorder?” Movius asked the rattled crowd afterward. “We can minimize sensory input. … That might be the difference between them being able to stay calm and them taking off.”
Movius, founder of the consultancy Autism Interaction Solutions, is one of a growing number of people around the U.S. working to teach law enforcement agencies to recognize autistic behaviors and ensure that encounters between neurodevelopmentally disabled people and law enforcement end safely.
She and City of Industry Mayor Cory Moss later passed out bags filled with tools donated by the city to aid interactions: a pair of noise-damping headphones to decrease auditory input, a whiteboard, a set of communication cards with words and images to point to, fidget toys to calm and distract.
“The thing about autistic behavior when it comes to law enforcement is a lot of it may look suspicious, and a lot of it may feel very disrespectful,” said Movius, who is also the parent of an autistic 25-year-old man. Responding officers, she said, “are not coming in thinking, ‘Could this be a developmentally disabled person?’ I would love for them to have that in the back of their minds.”
A sheriff’s deputy reads a pamphlet on autism during the training program.
(Genaro Molina / Los Angeles Times)
Autism spectrum disorder is a developmental condition that manifests differently in nearly every person who has it. Symptoms cluster around difficulties in communication, social interaction and sensory processing.
An autistic person stopped by police might hold the officer’s gaze intensely or not look at them at all. They may repeat a phrase from a movie, repeat the officer’s question or temporarily lose their ability to speak. They might flee.
All are common involuntary responses for an autistic person in a stressful situation, which a sudden encounter with law enforcement almost invariably is. To someone unfamiliar with the condition, all could be mistaken for intoxication, defiance or guilt.
Autism rates in the U.S. have increased nearly fivefold since the Centers for Disease Control began tracking diagnoses in 2000, a rise experts attribute to broadening diagnostic criteria and better efforts to identify children who have the condition.
The CDC now estimates that 1 in 31 U.S. 8-year-olds is autistic. In California, the rate is closer to 1 in 22 children.
As diverse as the autistic population is, people across the spectrum are more likely to be stopped by law enforcement than neurotypical peers.
About 15% of all people in the U.S. ages 18 to 24 have been stopped by police at some point in their lives, according to federal data. While the government doesn’t track encounters for disabled people specifically, a separate study found that 20% of autistic people ages 21 to 25 have been stopped, often after a report or officer observation of a person behaving unusually.
Some of these encounters have ended in tragedy.
In 2021, Los Angeles County sheriff’s deputies shot and permanently paralyzed a deaf autistic man after family members called 911 for help getting him to a hospital.
Isaias Cervantes, 25, had become distressed about a shopping trip and started pushing his mother, his family’s attorney said at the time. He resisted as two deputies attempted to handcuff him and one of the deputies shot him, according to a county report.
In 2024, Ryan Gainer’s family called 911 for support when the 15-year-old became agitated. Responding San Bernardino County sheriff‘s deputies shot and killed him outside his Apple Valley home.
Last year, police in Pocatello, Idaho, shot Victor Perez, 17, through a chain-link fence after the nonspeaking teenager did not heed their shouted commands. He died from his injuries in April.
Sheriff’s deputies take a trivia quiz using their non-writing hands, while wearing vision-distorting glasses, as Kate Movius, standing left, and Industry Mayor Cory Moss, right, ring cowbells. The idea was to help them understand the sensory overwhelm some autistic people experience.
(Genaro Molina / Los Angeles Times)
As early as 2001, the FBI published a bulletin on police officers’ need to adjust their approach when interacting with autistic people.
“Officers should not interpret an autistic individual’s failure to respond to orders or questions as a lack of cooperation or as a reason for increased force,” the bulletin stated. “They also need to recognize that individuals with autism often confess to crimes that they did not commit or may respond to the last choice in a sequence presented in a question.”
But a review of multiple studies last year by Chapman University researchers found that while up to 60% of officers have been on a call involving an autistic person, only 5% to 40% had received any training on autism.
In response, universities, nonprofits and private consultants across the U.S. have developed curricula for law enforcement on how to recognize autistic behaviors and adapt accordingly.
The primary goal, Movius told deputies at November’s training session, is to slow interactions down to the greatest extent possible. Many autistic people require additional time to process auditory input and verbal responses, particularly in unfamiliar circumstances.
If at all possible, Movius said, wait 20 seconds for a response after asking a question. It may feel unnaturally long, she acknowledged. But every additional question or instruction fired in that time — what’s your name? Did you hear me? Look at me. What’s your name? — just decreases the likelihood that a person struggling to process will be able to respond at all.
Moss’ son, Brayden, then 17, was one of several teenagers and young adults with autism who spoke or wrote statements to be read to the deputies. The diversity of their speech patterns and physical mannerisms showed the breadth of the spectrum. Some were fluently verbal, while others communicated through signs and notes.
“This population is so diverse. It is so complicated. But if there’s anything that we can show [deputies] in here that will make them stop and think, ‘Hey, what if this is autism?’ … it is saving lives,” Moss said.
Mayor Cory Moss, left, and Kate Movius hug at the end of the training program last November. Movius started Autism Interaction Solutions after her son was born with profound autism.
(Genaro Molina / Los Angeles Times)
Some disability advocates cautioned that it takes more than isolated training sessions to ensure encounters end safely.
Judy Mark, co-founder and president of the nonprofit Disability Voices United, says she trained thousands of officers on safe autism interactions but stopped after Cervantes’ shooting. She now urges families concerned about an autistic child’s safety to call an ambulance rather than law enforcement.
“I have significant concern about these training sessions,” Mark said. “People get comfort from it, and the Sheriff’s Department can check the box.”
While not a panacea, supporters argue that a brief course is better than no preparation at all. Some years ago, Movius received a letter from a man whose profoundly autistic son slipped away as the family loaded their car at the beach. He opened the unlocked door of a police vehicle, climbed into the back and began to flail in distress.
Though surprised, the officer seated at the wheel de-escalated the situation and helped the young man find his family, the father wrote to Movius. He had just been to her training.
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