Gov. Ron DeSantis on Friday revoked Disney’s particular tax privileges in Florida, culminating a unprecedented conflict between one of many Republican Occasion’s main figures and a strong firm with deep historic ties in his state.

The transfer, which reverses a 55-year association permitting the corporate to self-govern its theme park advanced, got here after a weekslong battle with Disney that grew to become an emblem of the nation’s broader cultural fights over training, sexuality and id.

The Florida standoff largely centered on an training legislation not too long ago signed by Mr. DeSantis. That legislation, referred to as the “Parental Rights in Schooling” measure — or, to its critics, “Don’t Say Homosexual” — prohibits classroom instruction about sexual orientation and gender id in some elementary faculty grades. After inner calls for to talk out, Disney, Florida’s largest non-public employer, had criticized the measure and paused political donations.

“I’m simply not snug having that sort of agenda get particular therapy in my state,” Mr. DeSantis stated on Friday, denouncing how Disney had responded to the training laws in addition to movies circulated within the conservative media that confirmed workers on the firm discussing issues of sexuality. A consultant for Disney declined to remark.

Broadly seen as retaliation, Mr. DeSantis’s transfer illustrated simply how drastically the G.O.P. has remodeled from the times when its leaders usually moved in lock step with the nation’s largest companies. The episode additionally confirmed how main corporations have felt rising strain to take a stand on heated political points.

During the last decade or so, and particularly after former President Donald J. Trump’s ascent, main Republicans have more and more seen political profit in criticizing company America.

“Republicans who for a very long time have had an in depth and heat relationship with U.S. companies have began to be extra selective about these relationships,” stated former Consultant Carlos Curbelo, a Florida Republican, including that the occasion’s lawmakers weren’t “afraid to confront even some common manufacturers that earlier than would have been unthinkable.”

He went on, “Whereas earlier than it could have been unimaginable {that a} politician, particularly a Florida politician, would confront Mickey Mouse, now there’s truly vital political incentive to take action.”

Mr. DeSantis, a potential 2024 presidential contender, has been on the forefront of nationwide battles over curriculums, school rooms and sports activities groups, points which have galvanized many conservative voters.

Democrats and advocacy organizations have been sharply essential of conservative efforts on these matters, and a handful of Republican lawmakers across the nation have questioned whether or not the flurry of legislative exercise on these topics is geared toward real-world issues.

L.G.B.T.Q. leaders and a few training officers have forcefully criticized the training legislation that set off the dispute in Florida, saying that it’s already having a chilling impact on academics and will do the identical for younger college students who’ve relied on faculties as a protected place to speak about private points.

“Governor DeSantis is wielding the facility of the state to punish companies merely for not falling according to his brutal and discriminatory assaults,” stated Joni Madison, the interim president of the Human Rights Marketing campaign, an L.G.B.T.Q. advocacy group. “Each step of this whole ordeal in Florida has been nothing greater than a shameful try to out-Trump Trump by means of concern, hate and lies.”

However for Republican politicians, the choice to tackle a significant firm over the problem is much less prone to backfire with at this time’s conservative base than in an earlier period when business-minded moderates had much more affect within the occasion.

“That is, in my opinion, a low-risk engagement right here in opposition to an organization that’s perceived by many Republican base voters to be aggressively pursuing or selling a coverage agenda,” Mr. Curbelo stated.

That shift, evident with the rise of the Tea Occasion throughout the Obama administration, plainly intensified amid the political realignment of the Trump period, as Republicans more and more grew to become the occasion of white working-class voters whereas some big-business and tech leaders — and lots of of their workers — moved left.

On the invoice signing on Friday, the place Mr. DeSantis additionally authorized measures together with the so-called Cease W.O.Okay.E. Act, which restricts how race is mentioned in faculties and companies, the governor blasted not solely Disney but in addition Google and Coca-Cola for what he forged as variety measures that have been overly ideological.

On the point out of Disney, the Florida crowd booed.

Patricia Mazzei contributed reporting.