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How Dad’s Fitness May Be Packaged and Passed Down in Sperm RNA | Quanta Magazine

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How Dad’s Fitness May Be Packaged and Passed Down in Sperm RNA | Quanta Magazine

In March 2025, in a preprint uploaded to biorxiv.org, Mansuy and colleagues reported that EVs in mice can transport certain RNAs, metabolites and lipids linked to early-life stress from circulating blood to sperm, with consequences for offspring. The offspring produced by these sperm cells had stress-related metabolic dysfunction as adults and bore the stress signatures in their own sperm RNA. “These changes imply a mechanistic link between sperm RNA modifications and phenotypic features in the offspring,” Mansuy’s team concluded in their paper, which has not yet been peer-reviewed.

Phenotypic Translation

Perhaps the trickiest step to understand is how sperm-borne molecules could influence an adult’s observable traits. In one form of experiment, researchers extract all the sperm RNA from mice that have been raised under stressful or health-altering conditions. Those isolated RNAs are then injected into a zygote. Pups that emerge usually “get the dad’s phenotypes,” Conine said, suggesting that the RNAs alone confer traits from dad to offspring.

But how? During early development, epigenetic processes reign. As one fertilized cell divides into two, and those cells divide again, and so on, one set of DNA instructions is dynamically and repeatedly reprogrammed. The growing body specializes into different cell types and is sculpted into a sequence of increasingly complex forms. It’s possible, then, that early epigenetic alterations to the genome could have significant downstream effects on an adult.

Research out of Conine’s lab, published in 2024, showed that sperm microRNAs alter gene expression in mouse embryos. Experiments like these, he said, support the idea that offspring can inherit paternal traits via the transfer of non-DNA molecular stowaways in sperm.

The recent Cell Metabolism paper took this idea a step further by tracing a mechanism by which this can happen. A team of more than two dozen Chinese researchers focused on the epigenetic transmission of exercise benefits, homing in on a set of microRNAs that reprogram gene expression in the early embryo. These changes ultimately result in skeletal muscle adaptations in adult offspring that enhance exercise endurance. The researchers found that well-exercised mice had more of these microRNAs in their sperm than sedentary mice did. When these microRNAs were transferred into zygotes, the adults they grew into were more physically fit, with more mitochondria in skeletal muscle and higher endurance.

But how did the molecules generate the exercise-positive phenotype? In experiments, the researchers found that the microRNAs suppressed a particular protein, which had the effect of boosting genes related to mitochondrial activity and metabolism.

Intriguingly, the sperm of physically trained male humans also hosted higher levels of many of the same microRNAs than those of untrained cohorts. “This cross-species conservation suggests a potential role for these sperm mi[cro]RNAs in intergenerational exercise adaptations in humans,” the researchers wrote.

The First Draft

The notion that a father’s lived experience can become recorded by his body, transmitted to his gametes and relayed to his offspring is no longer as outlandish as it once seemed. Many researchers in the field are willing to float speculative visions of what could be going on, even as they acknowledge that gaps remain.

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“Our hypothesis is that the epididymis ‘sees’ the world and alters the small RNAs it produces in response,” Rando said. “These RNAs are then delivered to the zygote upon fertilization and control early gene regulation and development to shape offspring health and disease.”

Conine speculates that once certain RNAs make their way into the egg, they trigger “a cascade of changes in developmental gene expression that then leads to these phenotypes” of the father showing up in the next generation. Remarkably, this unfolds even though the sheer volume of the sperm’s contents is so much less than an egg’s contents, including the relative amounts of RNA.

The full picture of how paternal experience and behavior might epigenetically influence offspring is not nearly in hand. Researchers are currently piecing the story together, one experiment at a time, rather than proving out every step sequentially in the same set of organisms. One of the gaps is in the characterization of what RNA and perhaps other epigenetic factors do in the zygote to modify genomic activity as it unfolds during development, Mansuy said.

“We are still blind men describing for the first time different parts of the same elephant,” Chen said. “The underlying mechanism is almost certainly an orchestra of a sperm RNA code and factors beyond that.”

Confirming the findings in humans would take enormous effort, but it would be key to turning these findings in mice into “informed medical advice,” Chen said. This would require well-controlled experiments following multiple generations, tracking diet, exercise, aging and environmental exposures, while also using advanced tools to decode sperm-packaged molecules — and then looking for strong correlations between the molecular and phenotypic data.

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Even amid the uncertainties, researchers are cautiously moving forward as they learn to believe the results of their own experiments. If they’re right, they will have discovered a new fact of life, Rando said. When he thinks about his two boys, he wonders what he might have done differently when he was younger, before they were born, that might have tweaked his RNA profile in ways that would affect them today.

“We don’t know enough yet to develop guidance like that,” Rando said. “Maybe we will get there.”

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Why 21-15-9 Might be the Smartest Workout Format in Fitness – and How to Use it to Drive Muscle Growth

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Why 21-15-9 Might be the Smartest Workout Format in Fitness – and How to Use it to Drive Muscle Growth

CrossFit means a lot of things to a lot of people – because it’s made up of a lot of things.

Since the rise of the fitness giant, countless brands, events and training methods have sprung up around it – not claiming to be CrossFit, but looking suspiciously CrossFit-esque.

There are, however, a handful of things that are uniquely CrossFit: the ‘Girls’ benchmark workouts. The Hero WODs and, of course, its signature rep schemes.

Chief among them is ’21-15-9′.

The 21-15-9 rep scheme may just be the single most CrossFit thing in existence. But what exactly is it? Where did it come from? And why might it actually be better at building muscle in a hurry than its conditioning roots would have you believe?

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Let’s have a look.

What Is 21-15-9?

If you’ve never encountered it before, the format couldn’t be simpler. Choose two exercises (occasionally more) and perform 21 reps of each, then 15 reps of each, then nine reps of each, completing the entire workout as quickly as possible – with good form.

Probably the best-known example is ‘Fran’: 21 thrusters and pull-ups, followed by 15 of each, then nine. On paper it doesn’t look especially intimidating. In practice, it’s one of the most feared benchmark workouts in fitness.

Where Did it Come From?

Unlike many modern training methods, 21-15-9 didn’t come out of a study. It came from the gym floor.

CrossFit founder Greg Glassman has explained that the format emerged through years of coaching and experimentation in the 1990s. Rather than chasing a perfect sets-and-reps prescription, he was looking for a workout that allowed athletes to maintain a high power output from start to finish.

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The thinking is surprisingly elegant. You begin with 21 reps while fresh. By the time you reach the set of 15, your ability to produce force has already fallen. By the final nine, you’re significantly more fatigued – but the workload has dropped by almost the same amount.

Instead of grinding through increasingly miserable sets of the same length, the workout ‘meets you where you are’, reducing the work required as your capacity declines. The result is a workout that encourages you to keep moving instead of standing around trying to recover.

The numbers themselves are also remarkably practical. Forty-five total reps per movement provides plenty of training volume without turning the session into an endurance slog, while every set divides neatly into thirds if you need to break it up.

(Although I’ve got to be honest, I’m a 20-15-10-5 man myself, just for the sake of round numbers.)

Why Does it Work So Well?

Although there isn’t research showing that 21-15-9 is somehow the magic formula, there are obvious reasons why it consistently produces brutally effective workouts.

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Descending reps help maintain intensity. As fatigue accumulates, reducing the target allows movement quality, bar speed and overall work rate to stay higher than they would if you simply repeated the same number of reps over and over.

It also tends to land in a physiological sweet spot. Most 21-15-9 workouts take between three and eight minutes, depending on the movements and the athlete. That’s long enough to create a serious cardiovascular challenge while still requiring meaningful force production throughout. You’re taxing your anaerobic systems hard while relying on your aerobic system to help you recover just enough to keep going.

Finally, there’s the psychological trick. The hardest-looking part comes first. Once you’ve survived the opening 21, every remaining round appears more manageable. ‘Only 15 left.’ Then, ‘Just nine.’ In reality, you’re becoming more fatigued with every rep, but the shrinking target keeps you attacking the workout instead of pacing too conservatively.

Why it Might be Surprisingly Good for Building Muscle

Perhaps the biggest misconception about 21-15-9 is that it’s ‘just cardio with weights’.

Choose the right load and something interesting happens. Very few athletes complete every round unbroken. Instead, the workout naturally evolves into a series of short, broken sets separated by only a few seconds of rest.

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Your 21 might become 11-5-5. Your 15 becomes 8-4-3. Your final nine might stay unbroken – or become 5-4.

In effect, you’ve accidentally turned the workout into a form of rest-pause training.

Those brief pauses allow just enough recovery to squeeze out more high-quality repetitions before fatigue catches up again. By the latter stages of each mini-set, you’re repeatedly working very close to failure, recruiting the high-threshold motor units with the greatest potential for muscle growth.

It’s a similar principle to rest-pause training, myo-reps and cluster sets: all methods used to accumulate hypertrophy-friendly volume while keeping the load relatively heavy and the rest periods brutally short.

You’re basically speed-running a large number of hard, growth-stimulating reps in a very small window of time. Could this help explain why elite CrossFit athletes often carry an impressive amount of muscle despite spending relatively little time performing traditional bodybuilding splits?

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It’s certainly plausible, although the ‘elite’ part often selects for athletes with the greatest muscle-building potential.

Much of their training isn’t simply conditioning. It’s high-density resistance training performed under accumulating fatigue, with only fleeting recovery between efforts. In other words, they’re often doing something bodybuilders have deliberately programmed for decades: packing a lot of hard work into a very short period of time.

That’s not to say 21-15-9 is superior to a well-designed hypertrophy programme. If your sole goal is building muscle, there are more efficient ways to do it.

But if you’re looking for a workout that develops fitness, tests your mettle and still provides a meaningful stimulus for strength and size, it’s easy to see why this deceptively simple rep scheme has remained one of CrossFit’s defining fingerprints for more than 20 years.

Best Bodyweight 21-15-9 Workout: ‘JT’

If you’re looking for an interesting twist on the 21-15-9 format, look no further than Hero WOD ‘JT’, which concentrates the muscle-building potential of the format into a brutal upper-body workout.

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Created in honour of Petty Officer 1st Class Jeff Taylor, who was killed in Afghanistan in 2006, the workout strips away barbells altogether and relies solely on three bodyweight movements:

21-15-9 reps of:

Don’t let the lack of equipment fool you. The volume – 45 reps of each movement, 135 reps in total – combined with the descending rep scheme makes this a brutal upper-body test, hammering the shoulders, chest and triceps while demanding serious muscular endurance.

Better still, it perfectly demonstrates one of the biggest strengths of 21-15-9. As fatigue mounts and the sets naturally fragment, the workout begins to resemble one giant rest-pause set, allowing you to accumulate a huge number of hard, near-failure reps in less than 10 minutes.

If your goal is building an impressive upper body while developing serious work capacity, there are few bodyweight workouts that deliver quite so much bang for your buck, making ‘JT’ one of my personal favourites.

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fitness magazine cover featuring a muscular man with kettlebells

If there’s one thing Kori Sampson knows, it’s how to optimise your body composition for performance. To tap into his knowledge as an elite athlete and coach, we asked him to create a 4-week plan to help you move faster, recover quicker and keep pushing when the fatigue sets in – all while improving your muscle-to-fat ratio.

Ready to build muscle, burn fat and come out the other side looking, feeling and performing better? Click here to get 14 days of free access to the plan via the Men’s Health app.


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10 minutes of swimming might not sound worth it – but I tried it for 2 weeks and found the benefits of a quick dip

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10 minutes of swimming might not sound worth it – but I tried it for 2 weeks and found the benefits of a quick dip

The concept of ‘exercise snacking’ has never been more popular. Not only is it convenient and accessible, but there is solid scientific evidence that short bursts of physical activity can yield real benefits for our health. But can a swimming workout be an effective ‘exercise snack’?

A study published in the European Heart Journal found that just 15 to 20 minutes of vigorous physical activity a week (almost as low as two minutes a day) was enough to significantly lower the risk of heart disease, cancer and early death. The study defined vigorous activity as any exercise that leaves you out of breath and raises your heart rate, including swimming.

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The Fitness Secrets of Wimbledon’s Top Tennis Pros

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The Fitness Secrets of Wimbledon’s Top Tennis Pros

While many of us are far from becoming top-ranked athletes, there’s plenty to learn from the pros when it comes to optimising our health and fitness. From Janik Sinnner’s muscle-building techniques to Novak Djokovic’s devotion to longevity, dig into these tennis pros’ secrets for peak performance.

Joris Verwijst/BSR Agency//Getty Images

CARLOS ALCARAZ

Fitness Game Changer:

Sand Footwork Drills

Any pro tennis player has to play with agility, but Alcaraz can move. To do so at a high level, the 21-year-old performs lateral movement drills in the sand, teaching his feet to drive up from an unstable surface. This can help prevent ankle injuries and build strength in his calves and shin muscles.

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jannik sinner

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JANNIK SINNER

Fitness Game Changer:

Landmine Rotations

Sinner has historically lacked the physical prowess of his competitors, so the 23-year-old has gone all in on strength and mobility work. He does landmine rotational exercises such as the hollow body landmine press, which builds upper-body power.

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novak djokavic

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NOVAK DJOKOVIC

Fitness Game Changer:

Devotion to Longevity

He’s been around this long for a reason. Djokovic, 37, eliminated gluten and dairy from his diet, started practising mindfulness techniques like conscious breathing and visualisation, and even brought a hyperbaric chamber to the 2019 US Open.

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ben shelton

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BEN SHELTON

Fitness Game Changer:

Explosive Strength Moves

Known for his consistently fast serves, Shelton, 22, relies on single-leg training, using dumbbells to do lateral lunges, step-ups, and even Bulgarian split squats. He focuses on exploding upward on every rep so he’s ready to attack the ball on each serve.

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frances tiafoe

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FRANCES TIAFOE

Fitness Game Changer:

Overcoming Isometrics

Tiafoe spent last off-season doing overcoming isometrics: exercises that force the 27-year-old to hold a position against a load he can’t move. This aids in boosting power and strength and can improve joint health.


fitness magazine cover featuring a muscular man with kettlebells

If there’s one thing Kori Sampson knows, it’s how to optimise your body composition for performance. To tap into his knowledge as an elite athlete and coach, we asked him to create a 4-week plan to help you move faster, recover quicker and keep pushing when the fatigue sets in – all while improving your muscle-to-fat ratio.

Ready to build muscle, burn fat and come out the other side looking, feeling and performing better? Click here to get 14 days of free access to the plan via the Men’s Health app.

Lettermark

Andrew Gutman, NASM-CPT is a journalist with a decade of experience covering fitness and nutrition. His work has been published in Men’s Health, Men’s Journal, Muscle & Fitness, and Gear Patrol. Outside of writing, Andrew trains in Brazilian Jiu-Jitsu, helps coach his gym’s kickboxing team, and enjoys reading and cooking. 

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